Assessment of genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) in zebrafish (<i>Danio rerio</i>) using the comet assay and micronucleus test

This study aimed to evaluate the genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-6) in fish. Adult zebrafish (Danio rerio) were exposed PBTA-6 solution 0.3 10 mg/L for 96 hr. Genotoxicity was evaluated using alkaline single-cell gel electrophoresis (comet assay) and micronucleus (MN) tests on blood gills. Although showed no positive response MN induction, it caused a concentration-dependent increase DNA damage. Fish (10 mg/L) hr followed by recovery period tap water also examined; damage decreased level before treatment within To compare cytochrome P450 (CYP) activity different tissues, CYP1A (ethoxyresorufin-O-deethylase; EROD methoxyresorufin-O-demethylase; MROD) CYP2B (penthoxyresorufin-O-depentylase; PROD benzyloxyresorufin-O-debenzylase; BROD) activities analyzed liver gill microsomes. The results that markedly induced gills damage, but show this could be reverted if fish are returned water.